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Biblioteca(s): |
Embrapa Rondônia. |
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Data corrente: |
30/12/2021 |
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Data da última atualização: |
01/01/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
SOUZA, J. A. de; SANTOS, M. R. A. dos. |
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Afiliação: |
JAQUELINE ANDRUCHEVITZ DE SOUZA, FIMCA; MAURICIO REGINALDO ALVES DOS SANTOS, CPAF-RO. |
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Título: |
Direct organogenesis and in vitro regeneration of plants of Piper marginatum. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
International Journal of Development Research, v. 11, n. 12, p. 52440-52442, Dec. 2021. |
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ISSN: |
2230-9926 |
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DOI: |
https://doi.org/10.37118/ijdr.23568.12.2021 |
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Idioma: |
Inglês |
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Conteúdo: |
Piper marginatum, popularly known in Brazil as Caapeba, is a native Amazonian plant of shrubby size, traditionally used for its active principles that act as therapeutic agents in wounds and pathologies. The objective of this work was to establish a protocol for the in vitro propagation of this species from leaf explants. For this, leaves were collected from two-year-old plants kept in a greenhouse at Embrapa Rondônia, in Porto Velho, Brazil. The leaves were subjected to disinfestation in a laminar flow chamber by immersion in 70% alcohol for one minute, followed by immersion in 2.0% sodium hypochlorite with 1.0 mL of Tween 20® for five minutes. Leaves were cut into 1.0 cm2 explants, which were inoculated into MS medium supplemented with 30.0 g L-1 sucrose, 6.0 g L-1 agar and variable concentrations of growth regulators benzylaminopurine (BA) (0.0, 1.13, 2.25 and 4.50 mg L-1) and 2,4-dichlorophenoxyacetic acid (2,4-D) (0.0, 1.11, 2.21 and 4.42 mg L-1) in factorial combination. The cultures were kept in a growth room at 25°C, under a 16-hour photoperiod. After 30 days, the number of shoots formed in the explants was evaluated. These shoots were subcultured on MS medium, under the conditions described, but without growth regulators. After 60 days, plantlet height, number of leaves, leaf area and root volume were evaluated. The treatment that contained 1.13 mg L-1 of BAP, in the absence of 2,4-D, resulted in the highest number of shoots and more vigorous plantlets after subculture, in relation to all evaluated variables. The plantlets were acclimatized in a greenhouse, with 50% shading and sprinkler irrigation three times a day, for their conversion into plants. MenosPiper marginatum, popularly known in Brazil as Caapeba, is a native Amazonian plant of shrubby size, traditionally used for its active principles that act as therapeutic agents in wounds and pathologies. The objective of this work was to establish a protocol for the in vitro propagation of this species from leaf explants. For this, leaves were collected from two-year-old plants kept in a greenhouse at Embrapa Rondônia, in Porto Velho, Brazil. The leaves were subjected to disinfestation in a laminar flow chamber by immersion in 70% alcohol for one minute, followed by immersion in 2.0% sodium hypochlorite with 1.0 mL of Tween 20® for five minutes. Leaves were cut into 1.0 cm2 explants, which were inoculated into MS medium supplemented with 30.0 g L-1 sucrose, 6.0 g L-1 agar and variable concentrations of growth regulators benzylaminopurine (BA) (0.0, 1.13, 2.25 and 4.50 mg L-1) and 2,4-dichlorophenoxyacetic acid (2,4-D) (0.0, 1.11, 2.21 and 4.42 mg L-1) in factorial combination. The cultures were kept in a growth room at 25°C, under a 16-hour photoperiod. After 30 days, the number of shoots formed in the explants was evaluated. These shoots were subcultured on MS medium, under the conditions described, but without growth regulators. After 60 days, plantlet height, number of leaves, leaf area and root volume were evaluated. The treatment that contained 1.13 mg L-1 of BAP, in the absence of 2,4-D, resulted in the highest number of shoots and more vigorous plantlets after subcult... Mostrar Tudo |
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Palavras-Chave: |
Piper marginatum; Protocolo de propagação. |
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Thesagro: |
Caapeba; Cultura In Vitro; Explante; Folha; Organogénese; Planta Medicinal; Propagação Vegetativa; Regeneração; Reprodução Vegetal. |
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Thesaurus NAL: |
Explants; Genetic techniques and protocols; In vitro regeneration; Leaves; Medicinal plants; Organogenesis; Plant reproduction; Vegetative propagation. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/229832/1/cpafro-18667.pdf
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Marc: |
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008 2021 bl uuuu u00u1 u #d
022 $a2230-9926
024 7 $ahttps://doi.org/10.37118/ijdr.23568.12.2021$2DOI
100 1 $aSOUZA, J. A. de
245 $aDirect organogenesis and in vitro regeneration of plants of Piper marginatum.$h[electronic resource]
260 $c2021
520 $aPiper marginatum, popularly known in Brazil as Caapeba, is a native Amazonian plant of shrubby size, traditionally used for its active principles that act as therapeutic agents in wounds and pathologies. The objective of this work was to establish a protocol for the in vitro propagation of this species from leaf explants. For this, leaves were collected from two-year-old plants kept in a greenhouse at Embrapa Rondônia, in Porto Velho, Brazil. The leaves were subjected to disinfestation in a laminar flow chamber by immersion in 70% alcohol for one minute, followed by immersion in 2.0% sodium hypochlorite with 1.0 mL of Tween 20® for five minutes. Leaves were cut into 1.0 cm2 explants, which were inoculated into MS medium supplemented with 30.0 g L-1 sucrose, 6.0 g L-1 agar and variable concentrations of growth regulators benzylaminopurine (BA) (0.0, 1.13, 2.25 and 4.50 mg L-1) and 2,4-dichlorophenoxyacetic acid (2,4-D) (0.0, 1.11, 2.21 and 4.42 mg L-1) in factorial combination. The cultures were kept in a growth room at 25°C, under a 16-hour photoperiod. After 30 days, the number of shoots formed in the explants was evaluated. These shoots were subcultured on MS medium, under the conditions described, but without growth regulators. After 60 days, plantlet height, number of leaves, leaf area and root volume were evaluated. The treatment that contained 1.13 mg L-1 of BAP, in the absence of 2,4-D, resulted in the highest number of shoots and more vigorous plantlets after subculture, in relation to all evaluated variables. The plantlets were acclimatized in a greenhouse, with 50% shading and sprinkler irrigation three times a day, for their conversion into plants.
650 $aExplants
650 $aGenetic techniques and protocols
650 $aIn vitro regeneration
650 $aLeaves
650 $aMedicinal plants
650 $aOrganogenesis
650 $aPlant reproduction
650 $aVegetative propagation
650 $aCaapeba
650 $aCultura In Vitro
650 $aExplante
650 $aFolha
650 $aOrganogénese
650 $aPlanta Medicinal
650 $aPropagação Vegetativa
650 $aRegeneração
650 $aReprodução Vegetal
653 $aPiper marginatum
653 $aProtocolo de propagação
700 1 $aSANTOS, M. R. A. dos
773 $tInternational Journal of Development Research$gv. 11, n. 12, p. 52440-52442, Dec. 2021.
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